[snetmcom]

seems like there is a surprising amount of misinformation. Thanks for clarifying.

[Gorgon_VZ]

Hi all, has anyone tested the performance of the novel chemistry yet? Any experiences regarding loading efficiency, readoutput, dataquality and so on? I would really, appreciate any kind of information from all you sequel users out there!

Thanks!

[DNATECH]

Hi All,
we have only run E.coli libraries with the new chemistry so far.

http://dnatech.genomecenter.ucdavis.edu/2016/11/10/new-service-long-read-sequencing-on-the-pacbio-sequel/

[sklages]

Have a look at https://downloads.pacbcloud.com/publ...opsisDemoData/

[Gorgon_VZ]

Hi all and thanks for your suggestions! I already recognised pacBios Arabidopsis release. However, the Sequel-data comes in a "new" format (*.bam) and one needs to use smrt-link to visualise the data. Samtools seems not to be feasible to have a look into the binary files. Is anyone aware of a workaround to open or even convert the files? Maybe to make them suitable for import into smrt-portal?
@DNATECH: Very helpful info!

[gconcepcion]

Hi all and thanks for your suggestions! I already recognised pacBios Arabidopsis release. However, the Sequel-data comes in a "new" format (*.bam) and one needs to use smrt-link to visualise the data. Samtools seems not to be feasible to have a look into the binary files. Is anyone aware of a workaround to open or even convert the files? Maybe to make them suitable for import into smrt-portal?
@DNATECH: Very helpful info!

What issue are you having with samtools?

the new SEQUEL *.bam files are 100% compatible with samtools, we routinely use it internally and it's included as part of SMRTLink

[spore]

Does anyone have any experience with the new Sequel chemistry on their own system?