HiSeq 2500 rapid run versus high output

kmkocot

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#1

HiSeq 2500 rapid run versus high output

11-21-2016, 02:36 PM

Hi all,

I'm taking advantage of an end-of-the-year RNA-Seq promotion from my sequencing provider. I was given the option of using their U.S. or overseas sequencing center. They said "if we were to do it here, we would be sequencing your samples on the HiSeq2500 in Rapid Mode rather than High Output mode." Apparently the turn-around time and data yield for each sample would be the same for either instrument. Any suggestions on which I should go after?

Best,
Kevin
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nucacidhunter

Jafar Jabbari

Join Date: Jan 2013

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11-21-2016, 05:51 PM

Average output of Rapid run is 150M/lane while HO is 250M and Gb of data from HO is cheaper than Rapid. Maybe they are selling reads not the lane. Data quality are similar so either should be fine.
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HiSeq 2500 rapid run versus high output

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