PacBio Sequel demultiplexing

[Ali May]

Hello,

Anyone who has experience with demultiplexing Sequel data via SMRT link command line options?

What I've done so far is to follow the "Barcode splitting for command-line analysis (SMRT Analysis v3.1)" section in

https://github.com/PacificBiosciences/PacBioFileFormats/wiki/BAM-recipes

and then use the demultiplex.py at https://github.com/PacificBiosciences/SMRT-Link

But when I do assembly with the demultiplexed data the results I get are just strange and not comparable to the assembly results I get from the same machine without demultiplexing. So I'm guessing that I'm doing something wrong.

Thanks,
Ali

[GenoMax]

Reach out to PacBio tech support directly. You may get a more rapid response that way. Sequel is so new that not many on this site have worked with that data as yet.

[Ali May]

I tried that already, have been waiting for three days now. I guess they're very busy for the upcoming software and chemistry release.

[GenoMax]

Ah well. People from PacBio do participate here so there is a chance you will see something before end of today (California, pacific time).

[phenotype]

Hi Ali,

We have a record of your case with PacBio tech support. It is case #52474 and was closed on 10/21.