Each MiSeq sequencing kit is for one run only and you can multiplex several libraries in one run depending on number of required reads. Multiplexing can be tailored to obtain equal or unequal number of reads for each library.
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NextSeq kit close to expire date
Does anybody know the different between NextSeq High output 300cycles and 150cycles kit? Only the reagent volume different? May I combine 2 of 150cycles kit for a 300 cycles run?Comment
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No, you couldn't. The NextSeq, like the MiSeq, has all reagents provided in a single slide-in cartridge. The instrument will read the barcode on the reagent cartridge and determine from that the number of cycles which that cartridge can be used for. There is no way to override this.Originally posted by GA-J View Post
[Disclaimer: My answer is not based on first hand knowledge but on assuming that the NextSeq operates like the MiSeq in this respect.]Comment
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The immune system’s power comes from its genetic diversity, allowing myriad threats to be neutralized through first recognizing foreign antigens. That diversity is also what makes the immune system so difficult to study. Recent advances in sequencing technology and computational biology, however, are giving researchers new tools to understand immune responses and immune-related diseases in greater detail.
This convergence of genetics, immunology, and computation...09-01-2026, 05:41 AM
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