rRNA derived cDNA removal after library generation

PigletIA

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Join Date: Apr 2011

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rRNA derived cDNA removal after library generation

01-11-2017, 11:25 AM

I have a specific application that requires ~5ug total fragmented RNA input for an RNA-IP step, then library generation and removal of the rRNA derived cDNA. I saw the Clontech Pico Input Mammalian Strand-Specific RNA-Seq Libraries for Illumina Platforms from Picogram Inputs of Total RNA has components called ZapR and R-Probe for doing this, I'm just wondering if there is a home-brew version I can use for a more flexible application.
Thanks!
Tags: rrna removal
HESmith

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#2

01-11-2017, 12:51 PM

See this publication.
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PigletIA

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#3

01-11-2017, 01:09 PM

Fantastic! Thanks a lot!
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