rRNA derived cDNA removal after library generation

PigletIA

Junior Member

Join Date: Apr 2011

Posts: 9
- Share
- Tweet
#1

rRNA derived cDNA removal after library generation

01-11-2017, 11:25 AM

I have a specific application that requires ~5ug total fragmented RNA input for an RNA-IP step, then library generation and removal of the rRNA derived cDNA. I saw the Clontech Pico Input Mammalian Strand-Specific RNA-Seq Libraries for Illumina Platforms from Picogram Inputs of Total RNA has components called ZapR and R-Probe for doing this, I'm just wondering if there is a home-brew version I can use for a more flexible application.
Thanks!
Tags: rrna removal
HESmith

Senior Member

Join Date: Oct 2009

Posts: 512
- Share
- Tweet
#2

01-11-2017, 12:51 PM

See this publication.
Comment
PigletIA

Junior Member

Join Date: Apr 2011

Posts: 9
- Share
- Tweet
#3

01-11-2017, 01:09 PM

Fantastic! Thanks a lot!
Comment

Previous template Next

Proteomic Platforms: How to Choose the Right Analytical Strategy to Improve Detection and Clinical Applications

by SEQadmin2

Proteomics platforms are evolving rapidly, with advances in mass spectrometry and affinity-based approaches expanding what researchers can detect and at what scale. As the field moves toward deeper proteome coverage and clinical applications, scientists face an increasingly complex landscape of tools. This article will explore how researchers are navigating these choices to find the right platform for their work.

The systematic characterization of the human proteome has...
- Channel: Articles
07-20-2026, 11:48 AM
Advanced Sequencing Platforms Tackle Neuroscience’s Toughest Genomics Problems

by SEQadmin2

Genomics studies in neuroscience face a special challenge due to the brain’s complexity and scarcity of samples. Mapping changes in cell type and state using conventional next-generation sequencing methods remains challenging. Advances in technologies like single-cell sequencing, spatial transcriptomics, and long-read sequencing have opened the door to deeper studies of the brain and diseases like Alzheimer’s, amyotrophic lateral sclerosis (ALS), and schizophrenia.
...
- Channel: Articles
07-09-2026, 11:10 AM
Cancer Drug Resistance: The Lingering Barrier to Rising Survival

by SEQadmin2

Cancer survival rates have significantly increased in the last few decades in the United States, reaching a combined 70% 5-year survival rate by 2021. Behind this number, there are years of research to find new therapies, drug targets, and early detection methods. But there is one core challenge that keeps slowing down these advances, and it’s about drug resistance.

There is no single reason why many patients don’t respond to treatment as expected. Cancer is...
- Channel: Articles
07-08-2026, 05:17 AM

Topics	Statistics	Last Post
Study Captures the First Moments of DNA Replication by SEQadmin2 Started by SEQadmin2, 07-24-2026, 12:17 PM	0 responses 30 views 0 reactions	Last Post by SEQadmin2 07-24-2026, 12:17 PM
Chemotherapy Leaves Detectable DNA Signatures in Childhood Tumors by SEQadmin2 Started by SEQadmin2, 07-23-2026, 11:41 AM	0 responses 23 views 0 reactions	Last Post by SEQadmin2 07-23-2026, 11:41 AM
Single-Cell Atlases Skew Toward European Ancestry, Analysis Finds by SEQadmin2 Started by SEQadmin2, 07-20-2026, 11:10 AM	0 responses 213 views 0 reactions	Last Post by SEQadmin2 07-20-2026, 11:10 AM
UC San Diego Bioengineers Map Gene Function in Human Stem Cells by SEQadmin2 Started by SEQadmin2, 07-13-2026, 10:26 AM	0 responses 79 views 0 reactions	Last Post by SEQadmin2 07-13-2026, 10:26 AM

Unconfigured Ad

rRNA derived cDNA removal after library generation

Comment

Comment

Latest Articles

ad_right_rmr

News