We currently use Illumina's ScriptSeq index primers for PCR amplifying cDNA produced by Wafergen's PrepX mRNA protocol. The ScriptSeq index primers only allow for 48 indexes. Are there any other primer sets with greater than 48 indexes that are compatible with Wafergen's PrepX protocol?
-
-
The ScriptSeq user guide provides the complete sequences of the reverse/index primers. If a user so desires, they can order their own custom primers from any good oligo/primer manufacturer and simply use index sequences not provided. Note that ScriptSeq uses indexes 17, 24 and 26 which are not "standard" illumina indexes of the same number (ILMN reserved those for the future). These indexes were designed prior to the purchase of Epicentre by Illumina. -
Yeah. I guess we could just make our own.
Thanks!Comment
-
Tumors contain complex mixtures of cell populations that evolve in response to treatment and host-specific factors. Tissue biopsies provide only static snapshots of a tumor’s state. But what if we could follow that evolution over time using a minimally invasive technique?
Liquid biopsies can detect tumor-derived material circulating in the bloodstream, providing a way to study treatment response, detect emerging resistance, and discover new biomarkers. Circulating...10-14-2026, 09:55 AM -
Researchers using sequencing and genomics tools often have to make trade-offs. They can choose between speed or scale, short reads or long-range information, or targeted panels or a view of the whole transcriptome. New technologies that have been released this year are built to address those tough choices.
We asked six companies the same four questions to learn about their latest products. The new technologies bring a lot to the table, including rethinking sequencing...
Comment