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  • MisUser
    Member
    • Jan 2017
    • 13

    #1

    Maximum number of cycles for kit Miseq 50 cycles

    Hi sequencing Masters!

    What is the maximum number of cycles we can do with a Miseq V2 50 cycles kit. I have been told that there is enough reagents for more than 55 cycles including the 6 cycles for index reading. Can someone confirm this information?
    Thank you very much for your support. It is very appreciated.
  • GenoMax
    Senior Member
    • Feb 2008
    • 7142

    #2
    How many are you looking to get?

    Comment

    • MisUser
      Member
      • Jan 2017
      • 13

      #3
      54 maximum + 6 for index

      Comment

      • GenoMax
        Senior Member
        • Feb 2008
        • 7142

        #4
        It should be fine. Local core does 66 cycle (50x8x8) runs off and on.

        Comment

        • MisUser
          Member
          • Jan 2017
          • 13

          #5
          Great news. Thank you for your answer Genomax!

          Comment

          • MisUser
            Member
            • Jan 2017
            • 13

            #6
            I got an answer from Illumina's tech support:
            MiSeq v2 contains enough reagents for 75 cycles of chemistry

            Comment

            • luobei
              Junior Member
              • Oct 2015
              • 1

              #7
              here you can find the answer:

              Comment

              • jkzebrafish
                Junior Member
                • Jan 2016
                • 3

                #8
                I know this is late but we routinely do 78 cycles with the MiSeq v2 50-cycle kit. (31 read1, 31 read2, 8 index1, 8 index2).

                Comment

                • nucacidhunter
                  Jafar Jabbari
                  • Jan 2013
                  • 1250

                  #9
                  Plus 7 dark cycles used for index2 read so 85 cycles in total. This seems to be 10 cycles more than Illumina specification.
                  Last edited by nucacidhunter; 10-18-2017, 09:30 PM.

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