MiSeq v3 600 Run Stopped at 301 cycle

[rahulrahi24]

The process cannot access the file 'D:\Illumina\MiSeqTemp\170210_M03340_0029_000000000-ATAVH\Recipe\RunState.xml' because it is being used by another process. (System.IO.IOException)

I was perfect loading cluster density of 1280

[GenoMax]

Have you contacted Illumina tech support? If they determine that this was a computer issue you would at least get replacement reagents for free.

[Markiyan]

IO.Exception-> Check the SMART's or your's HDD.

Contact the tech support, and

It looks like HDD problem to me.

Check the smart statuses of your MiSeq's HDD,
especially Reallocated Sectors Count and
Current Pending Sectors.

If any of then >0, than replace the HDD.

[rahulrahi24]

Thanks

Thanks Markiyan and GenoMax,

Illumina Tech Support said it might be because of Windows and Illumina software issues. They asked to run a system check and power cycle the instrument. They are replacing the kit.

I will check the SMARTs on HDD.


Thanks