Tweet
Setup sequence aligner in MPICH2 cluster
I am really new to cluster computer but I want to try it because using 1 computer take a bit of time. In my lab, I have several old PC so I think it would be good if I have a cluster computer. I think I will follow MPICH2 cluster in Ubuntu because it looks simple. My question is, can Bowtie2/BWA aligner be used from MPICH2 cluster? How to do it? Thank you.
-
-
You can actually try Rocks instead. Very few programs are truly parallel in NGS data analysis area so MPICH2 is not going to help.
-
Genomics studies in neuroscience face a special challenge due to the brain’s complexity and scarcity of samples. Mapping changes in cell type and state using conventional next-generation sequencing methods remains challenging. Advances in technologies like single-cell sequencing, spatial transcriptomics, and long-read sequencing have opened the door to deeper studies of the brain and diseases like Alzheimer’s, amyotrophic lateral sclerosis (ALS), and schizophrenia.
...Yesterday, 11:10 AM -
Cancer survival rates have significantly increased in the last few decades in the United States, reaching a combined 70% 5-year survival rate by 2021. Behind this number, there are years of research to find new therapies, drug targets, and early detection methods. But there is one core challenge that keeps slowing down these advances, and it’s about drug resistance.
There is no single reason why many patients don’t respond to treatment as expected. Cancer is...07-08-2026, 05:17 AM -
Love this - good data definitely starts from good input, and poor input can only give relatively poor data. I particularly like the mention of Nanodrop/absorbance based methods for quantification. It's such a toss up if you'll get an accurate reading or what amounts to a randomly generated number, and a lot of library/sequencing related issues can be traced back to poor quant.07-01-2026, 11:43 AM
Comment