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  • tfh4
    Junior Member
    • Feb 2017
    • 4

    #1

    Custom Yeast Genomic 70-mer Oligonucleotide Microarray chip NCBI GEO dataset?

    How to extract the numerical values for plotting time series curves for this Custom Yeast Genomic 70-mer Oligonucleotide Microarray chip NCBI GEO dataset?
    How to extract the numerical expression values for plotting time series curves for this Custom Yeast Genomic 70-mer Oligonucleotide Microarray NCBI GEO dataset?
    I would like to plot the mRNA expression time series curves based on the data of the following NcBI GEO dataset.
    Series GSE3705
    The title of this dataset is:
    JM43 cells grown aerobically in galactose and treated with Antimycin A
    The URL of this dataset is:

    The citation of the publication, which goes along with this dataset, is:
    Lai LC, Kissinger MT, Burke PV, Kwast KE. Comparison of the transcriptomic "stress response" evoked by antimycin A and oxygen deprivation in Saccharomyces cerevisiae. BMC Genomics 2008 Dec 23;9:627. PMID: 19105839.
    This publication is free available at https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2637875/
    My problem is that the platform is

    GPL1535 Custom Yeast Genomic 70-mer Oligonucleotide Microarray
    What do I need to do to extract the numerical gene expression values from this dataset, which are most meaningful for plotting time series curves?
    Thanks a lot
    Thomas

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