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  • gcR
    Member
    • Mar 2017
    • 15

    #1

    Looking for GTF file of exon annotations (GRCh38/hg38) without success

    Hello, Im new to the site and to be honest, to bioinformatics.

    I will sum it up, im having problems finding the correct file, although I passed the evening checking the forum, github, and other commonly used sources.

    I ended up dling from UCSC a table with exon, but Gene_id and transcript_id isnt giving me the gene names to make a merge with counts, and as far as I have been checking, noone had this issue.

    Code:
    chr1 hg38_knownAlt exon  X1048429  X1048578 X0.000000 X. .
    1 chr1 hg38_knownAlt exon   1048529   1048866         0  + .
    2 chr1 hg38_knownAlt exon  44040072  44040273         0  + .
    3 chr1 hg38_knownAlt exon 155187539 155191999         0  - .
    4 chr1 hg38_knownAlt exon 155189230 155190079         0  - .
    5 chr1 hg38_knownAlt exon 175097494 175117015         0  + .
    6 chr1 hg38_knownAlt exon 197102648 197143690         0  - .
                gene_id.altPromoter..transcript_id.altPromoter.
    1        gene_id bleedingExon; transcript_id bleedingExon; 
    2   gene_id bleedingExon; transcript_id bleedingExon_dup1; 
    3      gene_id strangeSplice; transcript_id strangeSplice; 
    4        gene_id cassetteExon; transcript_id cassetteExon; 
    5 gene_id strangeSplice; transcript_id strangeSplice_dup1; 
    6 gene_id strangeSplice; transcript_id strangeSplice_dup2;
    The parametres I used at UCSC were:
    Clade: Mammal
    Genome: Human
    Assambly: GRCH38/hg38
    track: UCSC Alt Events
    region: whole genome
    output: GTF

    If you could help me I would be grateful, Im not seeing the problem on my petition.

    Thanks in advance.

    GC.
    Beginner @ RNA-Seq, R programming, Linux, Python.-

    Please be patients!
  • GenoMax
    Senior Member
    • Feb 2008
    • 7142

    #2
    You can get the GTF files you need from GENCODE project here. Multiple options are available so choose what you need. If you truly want to remove other annotations except "exons" then you will need to do a bit of post-processing.
    Last edited by GenoMax; 03-29-2017, 12:05 PM.

    Comment

    • gcR
      Member
      • Mar 2017
      • 15

      #3
      Thanks you so much, sry for my late answer, I had some personal issues to attend but I got your answer immediatly and was much helpful.

      G.
      Beginner @ RNA-Seq, R programming, Linux, Python.-

      Please be patients!

      Comment

      • Geoffrey Buffington
        Junior Member
        • Apr 2020
        • 1

        #4
        cool broor thankks for sharing.

        Comment

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