Tweet
I am preparing total RNA samples from rat brain tissue to look at differential gene expression (single read, 1x50). HighSeq3000 allows for 300 million reads. I am wondering if I can use one lane for 23 samples, with the assumption that each sample will yield approximately 12 million reads.
-
-
Take a look at this thread for some background. Since HiSeq 3000 runs patterned flowcells you should consider this. Best option would be to dual index your samples and clean your libraries of adapter contamination.
12M reads per sample is not enough for DE with a mammalian genome.
-
Data variability is still an issue in sequencing technologies despite the advances in reproducibility and accuracy of these platforms. But the problem does not originate in the sequencing itself, but in the previous steps, before the sample reaches the sequencer.
The first step is collection, followed by preservation and sample preparation for analysis. Most scientists overlook those steps, but not being careful might just be skewing the experiment’s results.
...06-02-2026, 10:05 AM -
With the launch of new single-cell sequencing platforms in 2026, the field stands at an exciting inflection point. This article surveys the most impactful advances in the field and discusses how they’re reshaping research in cancer, immunology, and beyond.
Introduction
Single-cell sequencing technologies have undergone remarkable advances over the past decade, transitioning from low-throughput experimental approaches to highly scalable platforms capable of...05-22-2026, 06:42 AM
Comment