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  • sajeshpk
    Junior Member
    • Jun 2014
    • 6
    #61
    Hope you loaded same conc of lib in each SMRTs.As per our experience the data output depends on loading conc, SMRT lot,library quality( not applied in your case as you loaded same lib),laser,robotics etc even the quality of N2 you are using

    In your case what was the pore occupancy (p1,p2 and P0 values) for the SMRTs?

    Comment

    • BioKiwi
      Member
      • Sep 2014
      • 19
      #62
      One library, primer annealing, polymerase binding, clean up and Magbead binding was done in one batch (one tube) then was splitted into three wells on the same sample plate and sequenced using the same SMRT Cell tray and sequencing reagent. P0, P1 and P2% and output is different for each Cell. It seems that variation is result of what happens inside the sequencer or SMRT cell quality.

      Comment

      • sajeshpk
        Junior Member
        • Jun 2014
        • 6
        #63
        Hi
        What was the P0, P1 and P2 values for the cells and average readlength of each cell?

        Comment

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