Unconfigured Ad

Collapse
X
 
  • Time
  • Show
Clear All
new posts
  • neokao
    Member
    • Mar 2015
    • 30

    #1

    rnacocktail basic questions

    My lab typically does RNA-seq ~once a year and I do the bioinformatic analysis myself. So the annoying part for me being not the expert in routine NGS analysis from raw data, every time I get new RNA-seq raw data I almost have to redo the setup and software installation with some new (and maybe better) analysis tools developed and my working computer (MacOS) being upgraded.

    I have some basic concept of using Unix but yet I feel the most challenging part
    to me to to get these new softwares running correctly.

    This time, I decided to try out RNACocktail based on a Nature Communications paper.

    After some trial and error, I managed to get it installed on my Mac (MacOS 10.13).

    "pip install rnacocktail-0.2.1.tar.gz --user
    Processing ./rnacocktail-0.2.1.tar.gz
    Collecting pysam (from RNACocktail-Pipeline==0.2.1)
    Using cached pysam-0.12.0.1.tar.gz
    Collecting pybedtools (from RNACocktail-Pipeline==0.2.1)
    Using cached pybedtools-0.7.10.tar.gz
    Requirement already satisfied: six in /System/Library/Frameworks/Python.framework/Versions/2.7/Extras/lib/python (from pybedtools->RNACocktail-Pipeline==0.2.1)
    Installing collected packages: pysam, pybedtools, RNACocktail-Pipeline
    Running setup.py install for pysam ... done
    Running setup.py install for pybedtools ... done
    Running setup.py install for RNACocktail-Pipeline ... done
    Successfully installed RNACocktail-Pipeline-0.2.1 pybedtools-0.7.10 pysam-0.12.0.1"

    However, after I tried this:
    run_rnacocktail.py -h

    I got this error message:

    -bash: run_rnacocktail.py: command not found

    Any suggestions? Thanks.
    Last edited by neokao; 09-28-2017, 07:10 PM.

Latest Articles

Collapse

  • SEQadmin2
    16S/ITS Profiling vs. Shotgun Metagenomics: What Changes in Your Workflow?
    by SEQadmin2




    NGS has revolutionized clinical microbiology by offering new ways to identify and surveil pathogens without prior knowledge of the target. One popular approach is 16S rRNA and ITS amplicon sequencing, which targets specific microbial markers. But studies are increasingly incorporating shotgun metagenomics, a technique that sequences all DNA from a sample without targeting specific microbial markers. This approach allows more detailed taxonomic classification, detection...
    Today, 06:46 AM
  • SEQadmin2
    Multiomic Liquid Biopsy: What DNA/RNA Panels Reveal That DNA Alone Can’t
    by SEQadmin2



    Tumors contain complex mixtures of cell populations that evolve in response to treatment and host-specific factors. Tissue biopsies provide only static snapshots of a tumor’s state. But what if we could follow that evolution over time using a minimally invasive technique?

    Liquid biopsies can detect tumor-derived material circulating in the bloodstream, providing a way to study treatment response, detect emerging resistance, and discover new biomarkers. Circulating
    ...
    10-14-2026, 09:55 AM

ad_right_rmr

Collapse

News

Collapse

Topics Statistics Last Post
Started by SEQadmin2, 10-28-2026, 09:57 AM
0 responses
24 views
0 reactions
Last Post SEQadmin2  
Started by SEQadmin2, 10-28-2026, 09:51 AM
0 responses
15 views
0 reactions
Last Post SEQadmin2  
Started by SEQadmin2, 10-22-2026, 11:13 AM
0 responses
68 views
0 reactions
Last Post SEQadmin2  
Started by SEQadmin2, 10-22-2026, 10:48 AM
0 responses
48 views
0 reactions
Last Post SEQadmin2  
Working...