Single kit for bacterial RNAseq projects (from clinical samples)?

[seqtechno1]

Hello,

Does anyone know of a standalone kit or protocol which could deplete mammalian rRNA and bacterial rRNA to be left with bacterial RNA we want for sequencing? The best way I know how seems to be a convoluted mess of ribozero, microbe enrich, and truseq by illumina while skipping the poly-A selection step. Surely there has to be a more straightforward way to achieve desired RNAseq experiments for clinical bacterial samples?

[nucacidhunter]

If you want to use Truseq then you will need to do sequential depletion with microbial depletion reagent and use the eluate with RiboZero followed with the rest of protocol.

[ajeffs]

Ribo-Zero Epidemiology kit, which combines Ribo-zero human/mouse/rat and bacteria in a single tube for a single depletion step.