Yeah, we were quite surprised when I spoke to them and discovered this. In the future we might ask them to use all and increase the cycles to 14 or 15. That would explain A LOT of reasons why we are struggling with our samples (RNA, not so much for DNA).
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Hi jteeee2,
I just wanted to check back in with you and say thank you. We did some testing in the lab and both the primers / Taq you recommended appeared to work well. We submitted that to our Sequencing CORE and we were able to save over 2/3's of those 68 samples. That a huge relief!!! So instead of all 4 out of 72, we now have material for 49. We have a plan to save those other 23 samples and we are asking them to increase the cycles from 12 for future RNA/FFPE runs.
Thank you so much.
SueComment
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Glad to hear things are improving. Best of luck getting those last 23 samples made into libraries.Comment
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The immune system’s power comes from its genetic diversity, allowing myriad threats to be neutralized through first recognizing foreign antigens. That diversity is also what makes the immune system so difficult to study. Recent advances in sequencing technology and computational biology, however, are giving researchers new tools to understand immune responses and immune-related diseases in greater detail.
This convergence of genetics, immunology, and computation...09-01-2026, 05:41 AM
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