Recipe for NexteraXT respuspension buffer?

[Meyana]

So, I will be running out of resuspension buffer (RSB) for my NexteraXT kit before everything else...

Does anybody know what is in it or can guide me to some info on it?
Is it even advisable to make your own?

Thanks!

[nucacidhunter]

If you are not doing bead normalisation step, any common buffer such as TE, TE0.1 or EB will do it. For bead normalisation, EB will be better choice as its pH (8.0) is the same as RSB (RSB is proprietary).

[Meyana]

I will not do bead normalisation - I think I'll find some TE buffer

The TE0.1, what is the 0.1 in this regard?

[nucacidhunter]

It contains 0.1 mM EDTA in comparison to TE with 1 mM EDTA.

[Meyana]

Thanks for your help!