Hey all, I just found several of my RNA-Seq libraries have primer dimers, I was wondering what is the ratio of my final product that is now diluted in resuspension buffer to AMPure beads to get rid of the primer dimers?
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You will need to do a second clean up with the same bead/DNA ratio used for clean up if you have been following a kit protocol. You will need to do second clean up on all libraries to avoid batch affect. -
So I used trueseq, they recommend using 1:1 ratio, so can I proceed with 1:1Comment
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That should be fine, just make sure that your pipette is accurate and use the same pipette for measuring library volume and bead.Comment
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