Hello, is there a way to remove bacterial contamination from plant tissue before sequencing? We are planning on washing the tissue with weak bleach to kill the bacteria from the surface of the tissue. Any opinions on that? Or other suggestions? Thanks.
-
-
You can try agar embedding method (if interested read this study on this method). Also I know Cl2 gas for example is quite effective. Sure you can identify the bacteria and use antibiotic against the same, still be very cautious with it (here's an interesting study on it http://www.level.com.tw/html/ezcatfi...nation-COR.pdf)cheers,
engineering expert at yourhomeworkhelp.org. -
Thanks for infoComment
-
NGS has revolutionized clinical microbiology by offering new ways to identify and surveil pathogens without prior knowledge of the target. One popular approach is 16S rRNA and ITS amplicon sequencing, which targets specific microbial markers. But studies are increasingly incorporating shotgun metagenomics, a technique that sequences all DNA from a sample without targeting specific microbial markers. This approach allows more detailed taxonomic classification, detection...10-30-2026, 06:46 AM -
Tumors contain complex mixtures of cell populations that evolve in response to treatment and host-specific factors. Tissue biopsies provide only static snapshots of a tumor’s state. But what if we could follow that evolution over time using a minimally invasive technique?
Liquid biopsies can detect tumor-derived material circulating in the bloodstream, providing a way to study treatment response, detect emerging resistance, and discover new biomarkers. Circulating...10-14-2026, 09:55 AM
Comment