Fastqc

[salvadorherrandoperez]

Dear members.
I am using FASTQC* for the first time (in Galaxy).
Is there an online resource showing how to deal with a range of RNAseq quality issues to get a feel of how FASTQC works and could be applied to my data**.
Many thanks indeed.
Salva

* https://www.bioinformatics.babraham....ojects/fastqc/
** transcriptomes (wild reptile species in climate-change research) obtained through Ilumina sequencers.

[GenoMax]

Read these posts. Pay special attention to ones for duplicates, positional sequence bias at beginning of RNAseq reads.

[salvadorherrandoperez]

thanks

great resource - thanks.

[GenoMax]

Word of caution. Having a test of two "fail" (red X) in FastQC does not make your data automatically bad. If you have specific questions about anything post here with screenshots of FastQC data.