Merging PE reads before de novo RNA-Seq assembly

roussine

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Join Date: Nov 2017

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#1

Merging PE reads before de novo RNA-Seq assembly

01-25-2018, 08:39 AM

Hello!
can someone please give an opinion: does that make sense to merge PE reads before a de novo RNA-seq assembly (say with Trinity)?
Merging tools are quite fast (like USEARCH www.drive5.com/usearch/manual/merge_pair.html), and the amount of raw data is reduced drastically. Can merged data be suitable for further assembly in a “single-ends” mode, or am I missing some theory behind it?.. Seems like it may save a lot of computations.

Thanks in advance.
Leo
Tags: assembly, pe reads, rna-seq, trinity, usearch
GenoMax

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Join Date: Feb 2008

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#2

01-25-2018, 09:38 AM

For reference cross-posted to: https://www.biostars.org/p/295213/
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pmiguel

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#3

01-26-2018, 04:11 AM

I've never understood why people think that pair-merging prior to de novo assembly is a good idea. Seems like were that the case, assembly engines would just do pair-merging themselves prior to other assembly steps.

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Phillip
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Merging PE reads before de novo RNA-Seq assembly

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