Unconfigured Ad

Collapse
X
 
  • Time
  • Show
Clear All
new posts
  • Alarrisa
    Junior Member
    • Jan 2018
    • 1

    #1

    Handling NGS data using IGV

    Good evening,

    I do not work in bioinformatics, I have chosen a module in bioinformatics and completely out of my depth!
    I have an assessment where I am given a reference bacterial genome in FastA format and a GFF file containing the annotations and the position of predicted genes.
    I have illumine sequence reads generated by shotgun sequencing of genomes of several closely related bacteria.
    The objective: identify interesting differences among the sequenced bacterial genomes!
    I have no idea how to interpret this data. I started the module late due to personal reasons and I have 2 weeks to submit a poster with my findings!!

    Can one or a few of you bioinformatics wizards help me out please?
    Last edited by Alarrisa; 01-30-2018, 02:30 PM. Reason: it was wrong
  • GenoMax
    Senior Member
    • Feb 2008
    • 7142

    #2
    Can you tell us what you have learned as a part of this module? Do you know what the raw data looks like? What needs to be done with it in terms of QC/clean-up? Do you know what sequence alignment is with reference to NGS data? Are you limited to certain tools? Are you allowed to use online tools?

    Comment

    Latest Articles

    Collapse

    • SEQadmin2
      Multiomic Liquid Biopsy: What DNA/RNA Panels Reveal That DNA Alone Can’t
      by SEQadmin2



      Tumors contain complex mixtures of cell populations that evolve in response to treatment and host-specific factors. Tissue biopsies provide only static snapshots of a tumor’s state. But what if we could follow that evolution over time using a minimally invasive technique?

      Liquid biopsies can detect tumor-derived material circulating in the bloodstream, providing a way to study treatment response, detect emerging resistance, and discover new biomarkers. Circulating
      ...
      10-14-2026, 09:55 AM
    • SEQadmin2
      New Genomics Technologies Take Aim at Long-Standing Limits
      by SEQadmin2


      Researchers using sequencing and genomics tools often have to make trade-offs. They can choose between speed or scale, short reads or long-range information, or targeted panels or a view of the whole transcriptome. New technologies that have been released this year are built to address those tough choices.

      We asked six companies the same four questions to learn about their latest products. The new technologies bring a lot to the table, including rethinking sequencing
      ...
      09-28-2026, 10:25 AM

    ad_right_rmr

    Collapse

    News

    Collapse

    Topics Statistics Last Post
    Started by SEQadmin2, 10-15-2026, 10:52 AM
    0 responses
    19 views
    0 reactions
    Last Post SEQadmin2  
    Started by SEQadmin2, 10-14-2026, 05:39 AM
    0 responses
    26 views
    0 reactions
    Last Post SEQadmin2  
    Started by SEQadmin2, 10-13-2026, 09:56 AM
    0 responses
    22 views
    0 reactions
    Last Post SEQadmin2  
    Started by SEQadmin2, 10-09-2026, 11:14 AM
    0 responses
    28 views
    0 reactions
    Last Post SEQadmin2  
    Working...