Hi! I am trying to find the fastest sequencing machine. How long does the sequencing take at minimum? Does the bridge amplification, sequencing, or data processing take most time? Why does the sequencing take time, if there is only 250 bases to sequence (as all reads are read simultaneously)? Thanks in advance for the comments!
-
-
Minimum run time on Illumina systems is 4 hours on MiniSeq and MiSeq for 36 cycles. Generally, sequencing takes long time. In every cycles depending on 1, 2 or 4 colour chemistry few steps is required which includes base incorporation, imaging, debolcking and washing. YouTube search should find videos on different Illumina chemistries. One colour chemistry have slightly different workflow. -
the fastest sequencing time for an average run is probably Oxford Nanopore or Ion Torrent. Both of those systems do not use optical detection or enzymatic steps to build the sequences. I suppose you could say PacBio is rather quick as well, though it does use optics.Originally posted by Kultamuna2 View PostLast edited by snetmcom; 03-07-2018, 10:27 PM.Comment
-
Thanks for the answers! Some additional questions woke in my mind when I read the answer. So at fastest sequencing takes 4 hours, does it include also the data processing (genome assembly)? Is it usually included in these given sequencing times? Is this 4 hours for a human genome?Originally posted by nucacidhunter View Post
I have also heard that read length affects the sequencing time: shorter the read length, faster the sequencing. Why isn't then all sequencing carried out with this 36bp read length? Do we get lower quality data with lower read length?Last edited by Kultamuna2; 03-12-2018, 03:38 PM.Comment
-
Tumors contain complex mixtures of cell populations that evolve in response to treatment and host-specific factors. Tissue biopsies provide only static snapshots of a tumor’s state. But what if we could follow that evolution over time using a minimally invasive technique?
Liquid biopsies can detect tumor-derived material circulating in the bloodstream, providing a way to study treatment response, detect emerging resistance, and discover new biomarkers. Circulating...10-14-2026, 09:55 AM -
Researchers using sequencing and genomics tools often have to make trade-offs. They can choose between speed or scale, short reads or long-range information, or targeted panels or a view of the whole transcriptome. New technologies that have been released this year are built to address those tough choices.
We asked six companies the same four questions to learn about their latest products. The new technologies bring a lot to the table, including rethinking sequencing...
Comment