Unconfigured Ad

Collapse
X
 
  • Time
  • Show
Clear All
new posts
  • Aquilifer
    Junior Member
    • Apr 2018
    • 1

    #1

    Bowtie2 exact match and same length

    Hi,

    I have 8 fastq files with small RNA sequencing data and I would like to allign the sequences to a reference (.fa), thereby removing the sequences that do not allign perfectly to the reference sequences. However, the condition is that the perfect alligned seqeunces have the same length as the reference sequences to which they were alligned to. Example (not the real data, but to show what I mean):

    Ref sequence (.fa):
    CAATCGATCGATGCTAGTC

    sample sequences (.fastq):
    GCAATCGATCGATGC
    CAATCGATCGATGCTAGTC
    AATCGATCGATGCTAGTC
    GTACCATCGACT

    Expected output from bowtie (.sam):
    CAATCGATCGATGCTAGTC

    This is the command I used:
    bowtie2 -L 6 -i S,0,0.5 --rdg 1,6 --rfg 1,6 --norc --score-min C,0,-1 -p 8 -x "INDEX" (input_file.fastq) > (output_file.sam)

    The command runs and produces output, however not exactly what I expected.

    Bowtie2 output from command:
    CAATCGATCGATGCTAGTC
    AATCGATCGATGCTAGTC

    How can I change the bowtie2 command to remove perfect alligned sequences that are not the same length as the reference sequences? Or use Samtools to remove the smaller/longer sequences?

    Thanks in advance!
    Last edited by Aquilifer; 04-09-2018, 06:19 AM.

Latest Articles

Collapse

  • SEQadmin2
    Beyond CRISPR/Cas9: Understand, Choose, and Use the Right Genome Editing Tool
    by SEQadmin2



    CRISPR/Cas9 sparked the gene editing revolution for both research and therapeutics.1 But this system still showed severe issues that limited its applications. The most prominent were the heavy reliance on PAM sequences, delivery limitations, double-stranded breaks that prompt unintended edits and cell death, and editing inefficiency (both in targeting and in knock-in reliability).

    Despite this, “CRISPR helped turn genome editing from a specialized technique into
    ...
    Yesterday, 11:01 AM
  • SEQadmin2
    Proteomic Platforms: How to Choose the Right Analytical Strategy to Improve Detection and Clinical Applications
    by SEQadmin2


    Proteomics platforms are evolving rapidly, with advances in mass spectrometry and affinity-based approaches expanding what researchers can detect and at what scale. As the field moves toward deeper proteome coverage and clinical applications, scientists face an increasingly complex landscape of tools. This article will explore how researchers are navigating these choices to find the right platform for their work.

    The systematic characterization of the human proteome has
    ...
    07-20-2026, 11:48 AM
  • SEQadmin2
    Advanced Sequencing Platforms Tackle Neuroscience’s Toughest Genomics Problems
    by SEQadmin2



    Genomics studies in neuroscience face a special challenge due to the brain’s complexity and scarcity of samples. Mapping changes in cell type and state using conventional next-generation sequencing methods remains challenging. Advances in technologies like single-cell sequencing, spatial transcriptomics, and long-read sequencing have opened the door to deeper studies of the brain and diseases like Alzheimer’s, amyotrophic lateral sclerosis (ALS), and schizophrenia.
    ...
    07-09-2026, 11:10 AM

ad_right_rmr

Collapse

News

Collapse

Topics Statistics Last Post
Started by SEQadmin2, Yesterday, 02:55 AM
0 responses
8 views
0 reactions
Last Post SEQadmin2  
Started by SEQadmin2, 07-24-2026, 12:17 PM
0 responses
12 views
0 reactions
Last Post SEQadmin2  
Started by SEQadmin2, 07-23-2026, 11:41 AM
0 responses
12 views
0 reactions
Last Post SEQadmin2  
Started by SEQadmin2, 07-20-2026, 11:10 AM
0 responses
24 views
0 reactions
Last Post SEQadmin2  
Working...