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  • gbatzel
    Junior Member
    • Apr 2018
    • 1
    #1

    Correct Trimmomatic adapter sequence file to use?

    Hi,

    I was told by the sequencing core that my mRNA libraries were prepared with the TruSeq Dual index adapter. They gave me two different sequences:

    Index 1 (i7) Adapters GATCGGAAGAGCACACGTCTGAACTCCAGTCAC[i7]ATCTCGTATGCCGTCTTCTGCTTG

    Index 2 (i 5) Adapters AATGATACGGCGACCACCGAGATCTACAC[i5]ACACTCTTTCCCTACACGACGCTCTTCCGATCT

    I was wondering which Trimmomatic adapter .fasta file to use?
    Tags: None
  • GenoMax
    Senior Member
    • Feb 2008
    • 7142
    #2
    Use TruSeq3-PE.fa file.

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