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  • jlove
    Member
    • Apr 2011
    • 49
    #1

    FuPa reagent?

    Can anyone explain how the FuPa reagent works? In the context of Illumina library preparation, The FuPa reagent is a proprietary reagent that is used to partially digest the primers and phosphorylate the amplicons for adapter ligation. Illumina told me it was proprietary.

    Thanks in advance!
    Tags: None
    • torben
      Member
      • Oct 2012
      • 21
      #2
      Since it's proprietary no one outside Thermo really knows, but some good guesses can be found in this thread http://seqanswers.com/forums/showthread.php?t=27039

        Comment

        • r.rosati
          Member
          • Aug 2015
          • 95
          #3
          This patent record is a nice read regarding the FuPa reagent:
          US8728728B2 - Methods and compositions for multiplex PCR - Google Patents
          https://patents.google.com/patent/US8728728B2/en
          The present invention provides methods, compositions, kits, systems and apparatus that are useful for multiplex PCR of one or more nucleic acids present in a sample. In particular, various target-specific primers are provided that allow for the selective amplification of one or more target sequences. In one aspect, the invention relates to target-specific primers useful for the selective amplification of one or more target sequences associated with cancer or inherited disease. In some aspects, amplified target sequences obtained using the disclosed methods, kits, systems and apparatuses can be used in various downstream processes including nucleic acid sequencing and used to detect the presence of genetic variants.

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