gDNA library prep kit for NovaSeq 6000?

JakobHedegaard

Member

Join Date: Mar 2008

Posts: 62
Share

Post
#1

gDNA library prep kit for NovaSeq 6000?

05-18-2018, 02:03 AM

Going to have a NovaSeq installed soon and wonder which library prep kits are most optimal for human gDNA? We are going to use the libraries for both WGS and for capture (exome/panels). Index hoping must of course be handled by the library kit.
Thanks, /Jakob
Tags: gdna, library prep, novaseq
kwaraska

Senior Member

Join Date: Nov 2008

Posts: 131
Share

Post
#2

05-18-2018, 01:13 PM

novaseq

We use Illumina UDI for indexes as there are 96 choices so you can run a large number on the NovaSeq. In this way, if the forward index does not match the reverse index, you can discount the read as index hopping.

Depending on how many exomes you run, you may want to upgrade to the lane loader for the S4 flow cell so you can run as 4 lanes. That will give you more flexibility to run genomes and exomes on the NovaSeq. Otherwise, at standard coverage metrics, you will need a 384 index set.
Comment

Previous template Next

Beyond CRISPR/Cas9: Understand, Choose, and Use the Right Genome Editing Tool

by SEQadmin2

CRISPR/Cas9 sparked the gene editing revolution for both research and therapeutics.¹ But this system still showed severe issues that limited its applications. The most prominent were the heavy reliance on PAM sequences, delivery limitations, double-stranded breaks that prompt unintended edits and cell death, and editing inefficiency (both in targeting and in knock-in reliability).

Despite this, “CRISPR helped turn genome editing from a specialized technique into...
- Channel: Articles
07-31-2026, 11:01 AM
Proteomic Platforms: How to Choose the Right Analytical Strategy to Improve Detection and Clinical Applications

by SEQadmin2

Proteomics platforms are evolving rapidly, with advances in mass spectrometry and affinity-based approaches expanding what researchers can detect and at what scale. As the field moves toward deeper proteome coverage and clinical applications, scientists face an increasingly complex landscape of tools. This article will explore how researchers are navigating these choices to find the right platform for their work.

The systematic characterization of the human proteome has...
- Channel: Articles
07-20-2026, 11:48 AM
Advanced Sequencing Platforms Tackle Neuroscience’s Toughest Genomics Problems

by SEQadmin2

Genomics studies in neuroscience face a special challenge due to the brain’s complexity and scarcity of samples. Mapping changes in cell type and state using conventional next-generation sequencing methods remains challenging. Advances in technologies like single-cell sequencing, spatial transcriptomics, and long-read sequencing have opened the door to deeper studies of the brain and diseases like Alzheimer’s, amyotrophic lateral sclerosis (ALS), and schizophrenia.
...
- Channel: Articles
07-09-2026, 11:10 AM

Topics	Statistics	Last Post
Consortium Completes First Full Diploid Human Genome by SEQadmin2 Started by SEQadmin2, Yesterday, 07:41 AM	0 responses 12 views 0 reactions	Last Post by SEQadmin2 Yesterday, 07:41 AM
Long-Read Sequencing Closes Decades-Old Gaps in the Medaka Genome by SEQadmin2 Started by SEQadmin2, 08-03-2026, 10:13 AM	0 responses 27 views 0 reactions	Last Post by SEQadmin2 08-03-2026, 10:13 AM
New Genomic Method Uncovers Ancient Hominin DNA by SEQadmin2 Started by SEQadmin2, 07-31-2026, 02:55 AM	0 responses 39 views 0 reactions	Last Post by SEQadmin2 07-31-2026, 02:55 AM
Study Captures the First Moments of DNA Replication by SEQadmin2 Started by SEQadmin2, 07-24-2026, 12:17 PM	0 responses 25 views 0 reactions	Last Post by SEQadmin2 07-24-2026, 12:17 PM

Unconfigured Ad

gDNA library prep kit for NovaSeq 6000?

Comment

Latest Articles

ad_right_rmr

News