Has anyone here used the Opentrons OT-2 for library preparation? https://opentrons.com/ot-2
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"Accuracy Accurate down to 384-well plates"
This is a new measure of accuracy that I was previously unaware of.
Their only 'Sequencing Application' in their protocol library is for 16S. There are liquid handling platforms with a much wider array of NGS protocols ready to go than this.. -
A neighboring lab has the OT-One. This first version is unusable for just about anything - it can't hold the pipettes properly and the plates have a lot of play. Version two looks very different, though.Comment
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There have recently been a few discussions about them on Reddit; perhaps it might be a way to get in touch with people who use them.Comment
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We recently got an OT2. So far it works for single channel pipetting.
We have problems with both of our multi-channel pipettes.
The magnetic module seems to have a design flaw - the magnet is too close to the samples, even when disengaged and at maximum distance (achieved with an self-made adapter). Thus, the beads do not stay in suspension for long - ideally they should stay in suspension for 5 minutes before then engaging the magnet.Comment
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The immune system’s power comes from its genetic diversity, allowing myriad threats to be neutralized through first recognizing foreign antigens. That diversity is also what makes the immune system so difficult to study. Recent advances in sequencing technology and computational biology, however, are giving researchers new tools to understand immune responses and immune-related diseases in greater detail.
This convergence of genetics, immunology, and computation...09-01-2026, 05:41 AM
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