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  • Zero/1
    Junior Member
    • Feb 2018
    • 2

    #1

    How to resuspend Ampure beads which fail to resuspend?

    I drammatically overdried my Ampure beads, now even pipetting over and over I cannot resuspend them: the lie at the bottom of the tube in large clumps. Any idea? I am afraid I cannot mess this experiment ;(
  • luc
    Senior Member
    • Dec 2010
    • 469

    #2
    Give it more time and vortex once in a while. They will resuspend in the end.

    Comment

    • r.rosati
      Member
      • Aug 2015
      • 95

      #3
      I'm late to the party, but alternatively, wash with ethanol again and vortex well. The ethanol should remove most of the air trapped in the precipitate, and at that point you could dry again and resuspend.

      If you tried luc's suggestion, I'd be happy to know if it worked out too!

      Comment

      • Meyana
        Member
        • Sep 2017
        • 40

        #4
        I agree on just incubating longer - I put my samples on the thermomixer with intermittent mixing until they dissolve. It works fine

        Comment

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