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  • grace_pang
    Junior Member
    • Aug 2013
    • 4
    #1

    Adaptor length of Ligation Sequencing Kit 1D

    Does anyone know the sequence or at lease length of the adaptor from Nanopore Ligation Sequencing Kit 1D?

    I'm trying to figure out how much Ampure Beads to use to get rid of short fragments, so the length will be helpful.


    Thank you!
    Tags: None
  • wdecoster
    Member
    • Oct 2015
    • 97
    #2
    If you have access to the ONT community website you can find the adapter sequences here: https://community.nanoporetech.com/t...ing-kit-family

    They're about 60 nucleotides and 20 nucleotides (y-shaped). I probably cannot disclose the exact sequences.here.
    Last edited by wdecoster; 06-20-2018, 02:28 PM.

    Comment

    • grace_pang
      Junior Member
      • Aug 2013
      • 4
      #3
      Thank you wdecoster! Right now I couldn't login to the community because ONT messed up my login.. They are trying to figure out what went wrong..
      But the length is all what I want right now. Thank you!

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