Error in NEBNext RNA Library Prep: Samples With Different Number of PCR Cycles
Hey folks,
I'm new to RNA-seq, library prep, and this forum. I'm doing library prep right now and realized that some of my samples were amplified with 12 cycles of PCR, and a few were accidentally amplified with 15 cycles of PCR. Is there any way to control for different amounts of amplification when trying to measure differential expression? Or are those samples with 15 PCR cycles a lost cause? Any help would be greatly appreciated!
jlcoffin
I'm new to RNA-seq, library prep, and this forum. I'm doing library prep right now and realized that some of my samples were amplified with 12 cycles of PCR, and a few were accidentally amplified with 15 cycles of PCR. Is there any way to control for different amounts of amplification when trying to measure differential expression? Or are those samples with 15 PCR cycles a lost cause? Any help would be greatly appreciated!
jlcoffin
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