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  • ikripp
    Member
    • Jan 2018
    • 19

    #1

    MiSeq Error in LanePump

    Hi All,

    We just ran a MiSeq 2x300bp amplicon sequencing run over the weekend and it has completed with decent specs but has shown an error that says the following:
    "Error in LanePump. Syringe pump value failed to move. Valve overload. Command = '/1Qe\r', reply = 'j'.

    I've seen most other MiSeq errors before, and have already sent an email to illumina tech support. I was wondering if anyone else had experienced this before -if you have any insight into how it may have impacted the output etc.

    Thanks!
  • ikripp
    Member
    • Jan 2018
    • 19

    #2
    Update:
    Tech support have identified it as a software or hardware error and told me to power cycle.
    Unfortunately its a hardware error so now I have an engineer coming to fix the machine.

    Just in case it happens to anyone else!

    Comment

    • SwineFlu
      Junior Member
      • Nov 2017
      • 1

      #3
      Thanks ikripp,
      I got the exact problem few days ago and already called tech support. Just wonder to know how did they fix it - replacing the pump?

      Comment

      • ikripp
        Member
        • Jan 2018
        • 19

        #4
        Originally posted by SwineFlu View Post
        Thanks ikripp,
        I got the exact problem few days ago and already called tech support. Just wonder to know how did they fix it - replacing the pump?
        They ended up replacing the hard drive and that resolved the issue. I believe the pump error was a result of the hard drive failing. Tech support instructed me to power cycle the machine and when I did it wouldn't turn back on! Then they came and replaced the hard drive and we had to reconfigure the machine again.
        On a positive note it is now running smoothly!
        Last edited by ikripp; 08-16-2018, 10:59 PM.

        Comment

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