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  • smoics
    Member
    • Jan 2016
    • 15

    #1

    BluePippin use w/ Nextera Kits

    Always used BluePippin with XT kit, as a two-step size selection. Trying out the new Flex kit. Since the protocol calls for a set/given amount of beads in the Flex kit, will the BluePippin size selection be overkill? Has anyone used the two in combination? Does it work better or is the Flex kit best used alone? Thanks.
  • nucacidhunter
    Jafar Jabbari
    • Jan 2013
    • 1250

    #2
    Nextera DNA Flex workflow includes a double SPRI size selection. A Pippin size selection will reduce library diversity and is not required unless you have a specific application that needs tight size selection.

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