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  • cement_head
    Senior Member
    • Mar 2012
    • 265

    #1

    Non-Standard bases from RTA

    Weird question, does Illumina's current basecaller (RTA) on HiSeq3000 call non-standard bases? In other words, can you get something other than AGCT in the sequence read?
  • kmcarr
    Senior Member
    • May 2008
    • 1181

    #2
    Originally posted by cement_head View Post
    Weird question, does Illumina's current basecaller (RTA) on HiSeq3000 call non-standard bases? In other words, can you get something other than AGCT in the sequence read?
    Yes. An 'N'. Other than that no.

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