New job, desk includes a workstation with windows10. I've got WSL running for silly things like small qiime jobs, but wondering if anyone else is doing anything with WSL asides from replacing PuTTy as their client for SSHing into cluster?
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How did you get QIIME working? Is it version 1 or 2?Originally posted by ctseto View PostNew job, desk includes a workstation with windows10. I've got WSL running for silly things like small qiime jobs, but wondering if anyone else is doing anything with WSL asides from replacing PuTTy as their client for SSHing into cluster?
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In WSL, apt-get install qiime is QIIME 1.9.0Originally posted by Cistron View PostHow did you get QIIME working? Is it version 1 or 2?
I installed miniconda, then used the linux tutorial from the qiime2.org page.
You need Xming on your Win10 machine and a reasonably new version of Win10 (1803?). You'll want to use firefox on ubuntu. I think you need ubuntu-desktop and/or xubuntu-desktop; I'll have to find time for clean installs.
I got moving pictures to work with qiime view using ubuntu firefox, and it's a bit slow.
I may look into containers for fun, but...
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huh! I had no idea there was an actual debian package for QIIME available. I tried the conda install route, but USEARCH keeps throwing a core dump error when I test the installation.Originally posted by ctseto View PostIn WSL, apt-get install qiime is QIIME 1.9.0
I installed miniconda, then used the linux tutorial from the qiime2.org page.
You need Xming on your Win10 machine and a reasonably new version of Win10 (1803?). You'll want to use firefox on ubuntu. I think you need ubuntu-desktop and/or xubuntu-desktop; I'll have to find time for clean installs.
I got moving pictures to work with qiime view using ubuntu firefox, and it's a bit slow.
I may look into containers for fun, but...
Ok. I can only find version 1.8 through apt-search, but maybe that will do. Going through the installation now. Let's see how it goes.
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by GATTACATLove this - good data definitely starts from good input, and poor input can only give relatively poor data. I particularly like the mention of Nanodrop/absorbance based methods for quantification. It's such a toss up if you'll get an accurate reading or what amounts to a randomly generated number, and a lot of library/sequencing related issues can be traced back to poor quant.
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by SEQadmin2
I’m not a sequencing expert. I’m a purification scientist who uses NGS to evaluate workflows my group develops. With this perspective, we think about the sample first and the NGS workflow second. The sequencer is an exceptionally honest reporter, but it can only report on what you give it, so whether you get clean, interpretable data from an NGS workflow is largely determined before you begin.
Here are nine questions we think about, in roughly the order they matter, before...-
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