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Really dumb question, but did Qiagen revive pyrosequencing when I wasn't looking?
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Looks like they did. No obvious announcements/comments from pundits anywhere either (google does not seem to bring anything up other than Qiagen's own site). Must be an application specific release.
The PyroMark Q24 is highly suited for the analysis of CpG methylation, SNPs, insertion/deletions, STRs, variable gene copy number, as well as for microbial identification and resistance typing.Last edited by GenoMax; 12-18-2018, 08:54 AM. -
QIAGEN bought Biotage (what was Pyrosequencing AB and the original outlicencing entity for the IP) in 2008. The PyroMark assays have been available from QIAGEN for about that length of time from marketing materials. I'm not sure pyrosequencing ever really went away.Comment
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Thanks - so for these specific applications, it's still here.Originally posted by Bukowski View PostComment
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Love this - good data definitely starts from good input, and poor input can only give relatively poor data. I particularly like the mention of Nanodrop/absorbance based methods for quantification. It's such a toss up if you'll get an accurate reading or what amounts to a randomly generated number, and a lot of library/sequencing related issues can be traced back to poor quant.07-01-2026, 11:43 AM
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I’m not a sequencing expert. I’m a purification scientist who uses NGS to evaluate workflows my group develops. With this perspective, we think about the sample first and the NGS workflow second. The sequencer is an exceptionally honest reporter, but it can only report on what you give it, so whether you get clean, interpretable data from an NGS workflow is largely determined before you begin.
Here are nine questions we think about, in roughly the order they matter, before...
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