ChIPSeq sequencing depth for histone modifications
Hi,
We would like to sequence mouse Histone modification (H3K4me3,H3K4me1,H3K27me3,H3K27ac) chip-seq samples using illumina Hiseq2500. Previously we sequenced to depth of 40M reads/sample. And the number of enriched sites were good enough. Now, we would like to minimize the depth to 20M reads/sample. Is this sequencing depth (20Mreads) sufficient enough to analyze histone modification sites?
Looking forward for your inputs!
Thank you!
We would like to sequence mouse Histone modification (H3K4me3,H3K4me1,H3K27me3,H3K27ac) chip-seq samples using illumina Hiseq2500. Previously we sequenced to depth of 40M reads/sample. And the number of enriched sites were good enough. Now, we would like to minimize the depth to 20M reads/sample. Is this sequencing depth (20Mreads) sufficient enough to analyze histone modification sites?
Looking forward for your inputs!
Thank you!
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