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  • materan96
    Junior Member
    • Feb 2019
    • 1

    Demultiplex nanopore reads with custom barcodes

    We have a problem trying to demultiplex MinION sequences with custom barcodes. Do you have any software recommendations we can try for demultiplexing or how to demultiplex these custom barcodes with Albacore? We have tried using albacore but it only recognizes barcodes provided by Nanopore Technologies.
  • GenoMax
    Senior Member
    • Feb 2008
    • 7142

    #2
    If you know the barcode sequences you may be able to use bbduk.sh from BBMap suite to bin the reads you are interested in.

    Comment

    • Jugsy67
      Junior Member
      • Jul 2008
      • 2

      #3
      re Minion and multiples barcodes

      Hi Guys did this work i.e. using bbduk.sh from BBMap to bin the barcoded reads? We want to do the same for 16S rRNA gene amplicons and 96 barcodes on the MinIon.

      Comment

      • tickteng
        Junior Member
        • Apr 2019
        • 6

        #4
        how about porechop?

        Comment

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