Most Versatile DNA Prep Kit

seqsuave

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Join Date: May 2018

Posts: 9
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#1

Most Versatile DNA Prep Kit

03-14-2019, 12:50 PM

Hello Everyone!

I am interested in peoples' opinion on what they would consider to be the most versatile DNA prep kit/enzymes. Most DNA library prep methods are pretty consistent in terms of using the same general steps or similar enzymes. I am looking for a method that can be used for all kinds of library preps with only having to add or subtract one or two steps depending on the applications, ie. ChIPseq, Methylseq, WGS, Exome, ATACseq, Amplicon, etc.

The KAPA HyperPlus looks to be pretty versatile, but I wanted to get some input from other users. Let me know what you think.
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luc

Senior Member

Join Date: Dec 2010

Posts: 469
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#2

03-15-2019, 05:47 PM

I am afraid there is not one kit that is universally applicable.
ATACseq for example depends on the Nextera transposase.
ChIPseq will require adapter ligation.
Amplicon seq will vary a lot with the amplicon lengths and objectives.
....
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