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Hello people!
We have a sequencing application where we could really need, say, 50–100 more cycles from a 600-cycle MiSeq v3 kit.
Illumina states that the cartridge contains reagents for 625 cycles [1] (to account for dual indices we don't need, so 10–15 more cycles there already).
Then again, ECO got about 370 cycles from a 300 cycle kit [2] which is a fair bit more than the 325 stated by Illumina. Other people also mentioned that they pushed above the 25 reserve index cycles [3].
Lastly, this post explains how MadsAlbertsen got 600 cycles from a 400 cycle kit by filling up some wells with reagents from leftover cartridges.
So, my question: Does anybody has experience with increasing the cycle number on the 600-cycle v3 kits and can tell me what safely works? Either by experimenting how many possible cycles are in one cartrige or by filling up from an old cartridge.
We have a sequencing application where we could really need, say, 50–100 more cycles from a 600-cycle MiSeq v3 kit.
Illumina states that the cartridge contains reagents for 625 cycles [1] (to account for dual indices we don't need, so 10–15 more cycles there already).
Then again, ECO got about 370 cycles from a 300 cycle kit [2] which is a fair bit more than the 325 stated by Illumina. Other people also mentioned that they pushed above the 25 reserve index cycles [3].
Lastly, this post explains how MadsAlbertsen got 600 cycles from a 400 cycle kit by filling up some wells with reagents from leftover cartridges.
So, my question: Does anybody has experience with increasing the cycle number on the 600-cycle v3 kits and can tell me what safely works? Either by experimenting how many possible cycles are in one cartrige or by filling up from an old cartridge.
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