Ion PI™ Controls usage with Ion Torrent PGM

[Alex Alexl]

Hello,
Does anybody know about compatibility between control sphere from Ion PI™ Controls 200 Kit and PGM in terms of sphere size, sequencing primer and so on? I need a control sphere for PGM but it looks like there is just Ion PI control kit with premade libraries and spheres. Is there any other control sphere kit for PGM?

[r.rosati]

Hi,
you're right, the v3 version of the Ion PGM™ Controls Kit doesn't have any Control Ion Spheres. The v2 version did.
Well size is notably bigger in PGM chips compared to Proton's PI, so I would say that the ISPs are not interchangeable, unfortunately. You might end up losing them during the run, I suppose.
An aliquot of Control ISPs comes with PGM sequencing kits - although they are barely more than what's needed for the kit's normal usage.
Did you need to make a test run with only control ISPs, as opposed to the normal amount of control ISPs that go in a sequencing run? Thermo engineers do use full control runs to check the machines at installation, so perhaps Thermo might be able to help?

[Alex Alexl]

Thanks a lot. Yes I need to run full chip with control IPS.