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  • GW_OK
    Senior Member
    • Sep 2009
    • 411

    Short read 1 on a Nova

    Anyone tried a read 1 less than 25 cycles on a Nova? We've done it before on Miseq and Nextseq but not on Nova. I'm wondering if there's anything weird about the patterned flowcell that would make it not work....
  • GenoMax
    Senior Member
    • Feb 2008
    • 7142

    #2
    I don't immediately recall if we have done one on NovaSeq but have done so on patterned FC on HiSeq 4000. As long as you keep in mind that the quality scores are not properly calibrated until after cycle 25 it should work.

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