Any update on a possible merge with BioStar? They both seem to be continuing to cover very much the same material.
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No updates. Istvan and I have had a couple of conversations that started and ended positively, but with no concrete actions as of yet. I know he is (or was until very recently) out of the country so perhaps things will pick up again.Originally posted by wjeck View PostAny update on a possible merge with BioStar? They both seem to be continuing to cover very much the same material.
He has some good ideas about how to fund such a site, but that differ from how I've proceeded so far...so we have to reconcile those two things.
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There was a bit higher traffic...but unfortunately in my quest to fix things I 1.) forgot I should've linked to the QnA site in my forum closure message, and 2.) broke the OSQA install.Originally posted by bioinfosm View PostPssst Eco: with this seqanswers forum down, is there much higher activity on the Q n A site? Hint for getting higher activity for a couple of days, so more people have feedback :P
It would be an interesting experiment to try, but I'm still not convinced that the QnA can replace the forums.
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Hi Folks,
I have recently joint the forum, it is interesting. Actually i want to use Bowite Tool for 72 bp fragments of illumina read (microbail genome) assembling via windowXp based operating system. could any one guide me from very basics of window based installation and operation of this tool. Previously i have assemble a genome with DNAStar tool. Any body could help please. ?his/her help will be highly appreciated.
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Welcome, but please ask your question in the appropriate subforum if you are expecting a response.Originally posted by Asifullah View PostHi Folks,
I have recently joint the forum, it is interesting. Actually i want to use Bowite Tool for 72 bp fragments of illumina read (microbail genome) assembling via windowXp based operating system. could any one guide me from very basics of window based installation and operation of this tool. Previously i have assemble a genome with DNAStar tool. Any body could help please. ?his/her help will be highly appreciated.
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Req ChIP seq protocol
Hi guys, i am krishh working on miRNAs regulation in human cells.
I am doing some ChIP-seq these days, i need some help from one you guys regarding protocol. What are the conditions has to be really work to reduce adapters contamination for this new illumina plat form.
thanks in advance ..
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See the post right above yours for what I don't want to retype.Originally posted by roman View PostHi guys, i am krishh working on miRNAs regulation in human cells.
I am doing some ChIP-seq these days, i need some help from one you guys regarding protocol. What are the conditions has to be really work to reduce adapters contamination for this new illumina plat form.
thanks in advance ..
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Looks like you made a new post to me.Originally posted by unagaswamy View PostHi All,
I am new to this forum. I am working on validation of SNPs using NGS. I am not sure if this is the right place to post this. I am unable to make a new post. Can you please guide me on this?
UN
If you're trying to start a new thread, you can't in this subforum. Try one of the forums that are applicable to the topic of your question.
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I just signed up here and tried to ask a question, but there is no 'submit' button. There is a login/register button, but when I click that it seems to simply refresh the page. I think I need to reply to another thread in order to be able to ask a new question at all here. Is this true?
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Latest Articles
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by GATTACATLove this - good data definitely starts from good input, and poor input can only give relatively poor data. I particularly like the mention of Nanodrop/absorbance based methods for quantification. It's such a toss up if you'll get an accurate reading or what amounts to a randomly generated number, and a lot of library/sequencing related issues can be traced back to poor quant.
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Channel: Articles
07-01-2026, 11:43 AM -
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by SEQadmin2
I’m not a sequencing expert. I’m a purification scientist who uses NGS to evaluate workflows my group develops. With this perspective, we think about the sample first and the NGS workflow second. The sequencer is an exceptionally honest reporter, but it can only report on what you give it, so whether you get clean, interpretable data from an NGS workflow is largely determined before you begin.
Here are nine questions we think about, in roughly the order they matter, before...-
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