Unconfigured Ad

Collapse
X
 
  • Time
  • Show
Clear All
new posts
  • an_na
    Junior Member
    • Mar 2019
    • 2

    #1

    filtering variants with R

    Hi,

    I'm learning data analysis with R, using RStudio.
    I have my data in one file. I have variants from 3 tissue types, from 130 individuals. I would like to filter the variants within each individual, so that I can get: 1. unique variants for all 3 tissues, 2. variants common to all tissues, 3. variants common between 2 tissue types.
    The file that I'm working on has 118 columns (vcf annotated file), I would like to keep all columns.
    I've tried VennDiagram package, but it filters out variants that are present in any other individual, so in the overlapping files I loose some variants.
    I understand that I would need to use loop filtering, so that 1. I pick individuals by "ID" column, then sort variants by "variant" column and compare them between "tissue" column to output into all filtered types of variants (unique and common).

    Does anyone know how to do that???

    Cheers,
    A.
  • Melissa
    Senior Member
    • Aug 2008
    • 124

    #2
    To find overlapping variants between two files, you can use bcftools. If you want to generate a simple venn diagram, you can provide lists to http://bioinformatics.psb.ugent.be/webtools/Venn

    Comment

    Latest Articles

    Collapse

    • SEQadmin2
      16S/ITS Profiling vs. Shotgun Metagenomics: What Changes in Your Workflow?
      by SEQadmin2




      NGS has revolutionized clinical microbiology by offering new ways to identify and surveil pathogens without prior knowledge of the target. One popular approach is 16S rRNA and ITS amplicon sequencing, which targets specific microbial markers. But studies are increasingly incorporating shotgun metagenomics, a technique that sequences all DNA from a sample without targeting specific microbial markers. This approach allows more detailed taxonomic classification, detection...
      10-30-2026, 06:46 AM
    • SEQadmin2
      Multiomic Liquid Biopsy: What DNA/RNA Panels Reveal That DNA Alone Can’t
      by SEQadmin2



      Tumors contain complex mixtures of cell populations that evolve in response to treatment and host-specific factors. Tissue biopsies provide only static snapshots of a tumor’s state. But what if we could follow that evolution over time using a minimally invasive technique?

      Liquid biopsies can detect tumor-derived material circulating in the bloodstream, providing a way to study treatment response, detect emerging resistance, and discover new biomarkers. Circulating
      ...
      10-14-2026, 09:55 AM

    ad_right_rmr

    Collapse

    News

    Collapse

    Topics Statistics Last Post
    Started by SEQadmin2, 10-28-2026, 09:57 AM
    0 responses
    41 views
    0 reactions
    Last Post SEQadmin2  
    Started by SEQadmin2, 10-28-2026, 09:51 AM
    0 responses
    25 views
    0 reactions
    Last Post SEQadmin2  
    Started by SEQadmin2, 10-22-2026, 11:13 AM
    0 responses
    74 views
    0 reactions
    Last Post SEQadmin2  
    Started by SEQadmin2, 10-22-2026, 10:48 AM
    0 responses
    48 views
    0 reactions
    Last Post SEQadmin2  
    Working...