Does anyone know of a quicker alternative to PCR/agarose gel when QCing bisulphite conversion in large numbers of samples?
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I'm mostly posting to also follow this thread since i've also struggled with this.
I've tried the Qubit RNA kit, the zymo bisulfite converted control and just ballparking it based on the expected recovery %. Honestly, qPCR setup might be the best high throughput method for quantitation for you but it didn't quite fulfill all of my projects requirements
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The immune system’s power comes from its genetic diversity, allowing myriad threats to be neutralized through first recognizing foreign antigens. That diversity is also what makes the immune system so difficult to study. Recent advances in sequencing technology and computational biology, however, are giving researchers new tools to understand immune responses and immune-related diseases in greater detail.
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