Unconfigured Ad

Collapse
X
 
  • Filter
  • Time
  • Show
Clear All
new posts

  • Question: Multi omics dataset analyses, where to start? And methods to use?

    I have been given a dataset containing the following information: Species data was collected from Daphnia magna, the data consists of transcriptomic data (RNA-Seq) and polar metabolomic data (DIMS). This information was collected under 2 conditions, 1 = treatment with an unknown metal ion. 2 = treatment with an unknown metal nanoparticle. Data was collected over three time points; early (hours), intermediate, late (days). The experiment was repeated for multiple biological replicates. The task we have been given is to use statistics, machine learning, and bioinformatics knowledge to look for genes and metabolites that co-vary across conditions and in response to perturbations in an animal system.

    The data provided is in tsv format and contains the following: - Information on genes monitored including gene ID, gene family information, and information on functions. - Similar information for metabolites including empirical information, functional information and peaks in measurements. - Two other tsv files describing the change in gene expression over time for each individual and condition and another for metabolite data describing the same changes. This is all the information and instruction we have been given, we have 3 days to analyse the dataset. Could anyone suggest where to start looking for a method to start analysing this data? As we have covered limited machine learning, so our knowledge is limited at this time. Any advice on methods / resources would be greatly appreciated.

Latest Articles

Collapse

  • GATTACAT
    Reply to Nine Things a Sample Prep Scientist Thinks About Before Sequencing
    by GATTACAT
    Love this - good data definitely starts from good input, and poor input can only give relatively poor data. I particularly like the mention of Nanodrop/absorbance based methods for quantification. It's such a toss up if you'll get an accurate reading or what amounts to a randomly generated number, and a lot of library/sequencing related issues can be traced back to poor quant.
    07-01-2026, 11:43 AM
  • SEQadmin2
    Nine Things a Sample Prep Scientist Thinks About Before Sequencing
    by SEQadmin2


    I’m not a sequencing expert. I’m a purification scientist who uses NGS to evaluate workflows my group develops. With this perspective, we think about the sample first and the NGS workflow second. The sequencer is an exceptionally honest reporter, but it can only report on what you give it, so whether you get clean, interpretable data from an NGS workflow is largely determined before you begin.

    Here are nine questions we think about, in roughly the order they matter, before...
    06-18-2026, 07:11 AM

ad_right_rmr

Collapse

News

Collapse

Topics Statistics Last Post
Started by SEQadmin2, Yesterday, 11:08 AM
0 responses
7 views
0 reactions
Last Post SEQadmin2  
Started by SEQadmin2, 06-30-2026, 05:37 AM
0 responses
11 views
0 reactions
Last Post SEQadmin2  
Started by SEQadmin2, 06-26-2026, 11:10 AM
0 responses
19 views
0 reactions
Last Post SEQadmin2  
Started by SEQadmin2, 06-17-2026, 06:09 AM
0 responses
53 views
0 reactions
Last Post SEQadmin2  
Working...