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  • Gangcai
    Member
    • Nov 2009
    • 30

    strange bowtie index building and mapping problem

    Hello everyone,
    I have met a quite strange problem when running bowtie for my SOLID sequencing result.
    I have succeeded to build bowtie colorspace index for chimpanzee whole genome, but when I run bowtie , it showed the error like:
    "run_bowtie.sh: line 32: 1898 Segmentation fault $bowtie -C --phred33-quals -n 3 -a -m 3 --best --strata -p 4 -S $index -q $infile $out_dir/$out_name
    "
    Then I checked the output, the output just contain sam header and a few line of alignment result. The strange thing is that the sam header is like this(chromosome name is incorrect):
    @HD VN:1.0 SO:unsorted
    @SQ SN:IB<8D><EA>^RP<F3>{<<FD><FA><93>ދ<FC>^X%^M<C3>^R^V<DA>A LN:135001995
    @SQ SN:1 LN:8402541
    @SQ SN:2 LN:134204764
    @SQ SN:3 LN:8412303
    @SQ SN:4 LN:135371336
    @SQ SN:5 LN:2259969
    @SQ SN:6 LN:115868456
    @SQ SN:7 LN:9231045
    @SQ SN:8 LN:107349158
    @SQ SN:9 LN:2108736
    @SQ SN:10 LN:100063422
    @SQ SN:11 LN:3087076
    @SQ SN:12 LN:90682376
    @SQ SN:13 LN:6370548
    @SQ SN:14 LN:83384210
    @SQ SN:15 LN:5078517
    @SQ SN:16 LN:77261746
    @SQ SN:17 LN:1841920
    @SQ SN:18 LN:64473437
    @SQ SN:19 LN:2407237
    ......

    I have done same procedure for human, the sam header is :
    @HD VN:1.0 SO:unsorted
    @SQ SN:chr10 LN:135534747
    @SQ SN:chr11 LN:135006516
    @SQ SN:chr11_gl000202_random LN:40103
    @SQ SN:chr12 LN:133851895
    @SQ SN:chr13 LN:115169878
    @SQ SN:chr14 LN:107349540
    @SQ SN:chr15 LN:102531392
    @SQ SN:chr16 LN:90354753
    @SQ SN:chr17_ctg5_hap1 LN:1680828
    .....

    So it seems bowtie either didn't build a correct colorspace index or read the index incorrectly. By the way, I have checked the chimpanzee genome I used, it seems OK(the chromosome name is chr1,chr2a, .....chrX, ...).

    Have anybody met the same problem before? I am quite precious to hear your suggestion.

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