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  • geeneticode
    Junior Member
    • Aug 2020
    • 2
    #1

    Is a compositional bias in Sequence Content of initial bases in WES reads normal?

    Per Base Sequence Content

    Any experienced Whole Exome Sequencing analyst/experimentalist:

    the Per Base Sequence Content of the initial couple of bases from the 3' end of my WES reads have some type of compositional bias. Is this some "harmless" procedural or technical bias occurring during sequencing or is it something to be worried about for the downstream analysis?

    Attached Files
    Tags: fastqc, reads, wes, whole exome sequencing
    • HESmith
      Senior Member
      • Oct 2009
      • 512
      #2
      It's entirely normal and does not affect the results.

        Comment

        • geeneticode
          Junior Member
          • Aug 2020
          • 2
          #3
          Okay, good to know - thanks for your answer!

            Comment

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