Unconfigured Ad

Collapse
X
 
  • Filter
  • Time
  • Show
Clear All
new posts
  • Kaskere
    Junior Member
    • Jul 2018
    • 7

    guzip paired fast.gz files

    Hello,
    I have a bunch of paired fastq.gz files, and I need to decompress them for further low quality data trimming.
    However gunzip command is not working on paired files. Any suggestions?

    this is the error output
    gzip: file.fastq.gz: unexpected end of file

    Thank you!
    Last edited by Kaskere; 09-01-2020, 01:36 AM.
  • GenoMax
    Senior Member
    • Feb 2008
    • 7142

    #2
    Are you sure the files are gzipped (is spite of what the extension says)? What does

    Code:
     file one_file.fastq.gz
    say?

    Comment

    • Kaskere
      Junior Member
      • Jul 2018
      • 7

      #3
      Originally posted by GenoMax View Post
      Are you sure the files are gzipped (is spite of what the extension says)? What does

      Code:
       file one_file.fastq.gz
      say?
      myfile.fastq.gz: gzip compressed data, from FAT filesystem (MS-DOS, OS/2, NT)

      they are compressed..

      These paired end files were obtained after PE Trimmomatics

      Comment

      • Biocomputronics
        Junior Member
        • Sep 2017
        • 6

        #4
        Try using "zcat" to see if it can display the file contents like:

        zcat file.fastq.gz

        If it displays the file contents, then redirect the output to a new file like:

        zcat file.fastq.gz > new_file.fastq

        Comment

        • GenoMax
          Senior Member
          • Feb 2008
          • 7142

          #5
          @kaskere: Were these files compressed using Windows zip program? You may need to use "-S .zip" option for gunzip.

          Comment

          Latest Articles

          Collapse

          • SEQadmin2
            Advanced Sequencing Platforms Tackle Neuroscience’s Toughest Genomics Problems
            by SEQadmin2



            Genomics studies in neuroscience face a special challenge due to the brain’s complexity and scarcity of samples. Mapping changes in cell type and state using conventional next-generation sequencing methods remains challenging. Advances in technologies like single-cell sequencing, spatial transcriptomics, and long-read sequencing have opened the door to deeper studies of the brain and diseases like Alzheimer’s, amyotrophic lateral sclerosis (ALS), and schizophrenia.
            ...
            07-09-2026, 11:10 AM
          • SEQadmin2
            Cancer Drug Resistance: The Lingering Barrier to Rising Survival
            by SEQadmin2



            Cancer survival rates have significantly increased in the last few decades in the United States, reaching a combined 70% 5-year survival rate by 2021. Behind this number, there are years of research to find new therapies, drug targets, and early detection methods. But there is one core challenge that keeps slowing down these advances, and it’s about drug resistance.

            There is no single reason why many patients don’t respond to treatment as expected. Cancer is...
            07-08-2026, 05:17 AM
          • GATTACAT
            Reply to Nine Things a Sample Prep Scientist Thinks About Before Sequencing
            by GATTACAT
            Love this - good data definitely starts from good input, and poor input can only give relatively poor data. I particularly like the mention of Nanodrop/absorbance based methods for quantification. It's such a toss up if you'll get an accurate reading or what amounts to a randomly generated number, and a lot of library/sequencing related issues can be traced back to poor quant.
            07-01-2026, 11:43 AM

          ad_right_rmr

          Collapse

          News

          Collapse

          Topics Statistics Last Post
          Started by SEQadmin2, 07-13-2026, 10:26 AM
          0 responses
          15 views
          0 reactions
          Last Post SEQadmin2  
          Started by SEQadmin2, 07-09-2026, 10:04 AM
          0 responses
          29 views
          0 reactions
          Last Post SEQadmin2  
          Started by SEQadmin2, 07-08-2026, 10:08 AM
          0 responses
          16 views
          0 reactions
          Last Post SEQadmin2  
          Started by SEQadmin2, 07-07-2026, 11:05 AM
          0 responses
          33 views
          0 reactions
          Last Post SEQadmin2  
          Working...