Tweet
There appears to be a lot of excitement about the Marco Island conference this year? Are there any Seqanswers members attending who can update the rest of us?
-
-
Rock on, I was just about to post this. I will put this out on the RSS feed and see if we can drag some people in!
Hope you don't mind if I edit your title before I put it out there!
-
-
-
Have Agilent announced their array enrichment plans yet? I hear it's nearly kitted and ready for release?Comment
-
-
What is kitted? The long oligos for the whole exonome? I think Nimblegen is more ready to release their "kit".Originally posted by ECO View PostComment
-
Sorry. I meant that some information I have (via Agilent reps) tells me that they are about to release an enrichment methodology that has been refined to the degree that it will be available in "kit" format (ie...reagents plus enrichment oligo sets).Originally posted by seqer View Post
It was a big secret from Agilent reps, that would be annouced at Marco Island.Comment
-
Thanks ECO! Has anyone heard anything from agilent at AGBT? If anyone could give a brief summary of AGBT, that would be really awesome!Originally posted by ECO View PostComment
-
Just found the following here:
So it's a solution phase capture array. Figured they would move away from that Church gap-filling/circularization method.“We prepare a labeled derivative of Agilent’s oligo libraries to use for direct capture of target sequences by hybridization. The method is highly multiplexed, with a simple, efficient and inexpensive laboratory process, thus overcoming the sample preparation bottleneck.”
Seems like this will make hybridization time and cost much less than a Nimblegen capture array, especially if they amplify the pool of oligo's after they are cleaved from the chip.
Comment
-
PacBio Highlight
The highlight of AGBT had to be the first public presentation from Steven Turner at Pacific Biosciences - a fitting way to close a great meeting. Here's more on what young Steve and his PacBio pals are up to:
Kevin Davies
Author, The $1,000 GenomeComment
-
Has anyone announced dates for AGBT 2009?Comment
-
Kevin Davies
Author, The $1,000 GenomeComment
-
Love this - good data definitely starts from good input, and poor input can only give relatively poor data. I particularly like the mention of Nanodrop/absorbance based methods for quantification. It's such a toss up if you'll get an accurate reading or what amounts to a randomly generated number, and a lot of library/sequencing related issues can be traced back to poor quant.07-01-2026, 11:43 AM
-
I’m not a sequencing expert. I’m a purification scientist who uses NGS to evaluate workflows my group develops. With this perspective, we think about the sample first and the NGS workflow second. The sequencer is an exceptionally honest reporter, but it can only report on what you give it, so whether you get clean, interpretable data from an NGS workflow is largely determined before you begin.
Here are nine questions we think about, in roughly the order they matter, before...
Comment